Difference between revisions of "Part:BBa K814000"
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This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli. | This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli. | ||
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+ | [[Image:Dhqs.png]] | ||
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+ | Figure 1. Colony PCR screen confirming cloning of DHQS into E. coli pUCBB plasmid. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 22:27, 13 October 2012
dehydroquinate synthase (DHQS) generator
Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate.
This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli.
Figure 1. Colony PCR screen confirming cloning of DHQS into E. coli pUCBB plasmid.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 250
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 250
Illegal NotI site found at 1373 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 250
Illegal BglII site found at 134
Illegal XhoI site found at 1381 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 250
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 250
- 1000COMPATIBLE WITH RFC[1000]