Difference between revisions of "Part:BBa K779307"
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<i> We transfected 250 ng of Hef1A:TagBFP into 100,000 HEK293 cells using Lipofectamine 2000 reagent. Images were taken on a Leica Confocal microscope at 10X. Left shows a brightfield image. Right shows the blue channel </i>
  
 
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Revision as of 03:28, 11 October 2012

TagBFP-FF5 MammoBlock We appended microRNA binding sites to TagBFP, an optimized Blue Fluorescent Protein.
Tagchar.png
We transfected 250 ng of Hef1A:TagBFP into 100,000 HEK293 cells using Lipofectamine 2000 reagent. Images were taken on a Leica Confocal microscope at 10X. Left shows a brightfield image. Right shows the blue channel

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 89
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 722