Difference between revisions of "Part:BBa K779301"
 
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<b> Testing eYFP driven by a Hef1A promoter (BBa_K779200) </b>
 
<b> Testing eYFP driven by a Hef1A promoter (BBa_K779200) </b>
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[[Image:EYFPchar.png]]
 
[[Image:EYFPchar.png]]
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<i> This image was taken on an Evos microscope at 10X. On the left, there is a brightfield image of 200,000 cells transiently transfected with Lipofectamine 2000 with 500 ng of Hef1A:eYFP. On the right, is an image taken in the FITC channel and we see high transfection efficiency and eYFP expression 48 hours post-transfection. </i>
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 02:23, 11 October 2012

eYFP MammoBlock

An engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces yellow.

Testing eYFP driven by a Hef1A promoter (BBa_K779200)
EYFPchar.png
This image was taken on an Evos microscope at 10X. On the left, there is a brightfield image of 200,000 cells transiently transfected with Lipofectamine 2000 with 500 ng of Hef1A:eYFP. On the right, is an image taken in the FITC channel and we see high transfection efficiency and eYFP expression 48 hours post-transfection.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 211
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]