Difference between revisions of "Part:BBa K732000:Experience"

Revision as of 04:20, 10 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K732000

E. coli were transformed with pET30a vector containing His-tagged hCG-β under the control of a strong promoter and the lac operator. In the basal state, lacI repressor binds to the operator and blocks transcription of hCG-β. Cells were induced with IPTG, which causes a conformational change in the repressor that unblocks transcription.

Folded protein mixture was applied to a pregnancy test kit, and the control band appeared. The test strip was allowed to sit overnight, and by the next day the test band had appeared, demonstrating detection of hCG-β.

Test band was not observed when extract was applied without folding solution.

User Reviews

UNIQeef4cc7f35863298-partinfo-00000000-QINU UNIQeef4cc7f35863298-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
@@ Line 5: / Line 4: @@
 ===Applications of BBa_K732000===
+''E. coli'' were transformed with pET30a vector containing His-tagged hCG-β under the control of a strong promoter and the lac operator. In the basal state, lacI repressor binds to the operator and blocks transcription of hCG-β. Cells were induced with IPTG, which causes a conformational change in the repressor that unblocks transcription.
+Folded protein mixture was applied to a pregnancy test kit, and the control band appeared. The test strip was allowed to sit overnight, and by the next day the test band had appeared, demonstrating detection of hCG-β.
+Test band was not observed when extract was applied without folding solution.
+[[Image:Virginia_2012_Aug_WT-HCG_Preg_Test.jpg]]
 ===User Reviews===