Difference between revisions of "Part:BBa K783067"
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This is an inverter with pBad driving tetR with GFP as a reporter. pTetR has RFP has a reporter. We used a weaker RBS (B0032) to control the tetR expression and it helped the inverter function properly. | This is an inverter with pBad driving tetR with GFP as a reporter. pTetR has RFP has a reporter. We used a weaker RBS (B0032) to control the tetR expression and it helped the inverter function properly. | ||
| − | We used flow cytometry to measure the function of our inverter. | + | We used flow cytometry to measure the function of our inverter. As arabinose concentration increases, GFP also increases. The increase of arabinose also increases the level of tetR, which can be seen as the RFP decreases under the control of the pTetR. |
[[image:inverter.png|550px|center]] | [[image:inverter.png|550px|center]] | ||
Revision as of 01:45, 10 October 2012
pBad-pTetR inverter
This is an inverter with pBad driving tetR with GFP as a reporter. pTetR has RFP has a reporter. We used a weaker RBS (B0032) to control the tetR expression and it helped the inverter function properly.
We used flow cytometry to measure the function of our inverter. As arabinose concentration increases, GFP also increases. The increase of arabinose also increases the level of tetR, which can be seen as the RFP decreases under the control of the pTetR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1247
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1187
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 3490
Illegal AgeI site found at 3602 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2634
Illegal SapI site found at 961