Difference between revisions of "Part:BBa K783054"
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It has two MoClo fusion sites flanking it: 5' C [part] D 3' | It has two MoClo fusion sites flanking it: 5' C [part] D 3' | ||
| − | The fusion site letters refer to 4bp fusion sites: A = GGAG | + | The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. |
| + | |||
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Latest revision as of 19:17, 7 October 2012
This is a MoClo converted version of BBa_C0079
This is a MoClo converted version of BBa_C0079, which is the coding region for the lasR protein, which accepts chemical signal AI-1 (made by lasI protein).
It has two MoClo fusion sites flanking it: 5' C [part] D 3'
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 322
Illegal AgeI site found at 519 - 1000COMPATIBLE WITH RFC[1000]