Difference between revisions of "Part:BBa K779502"
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'''IDT DNA Oligo Order:''' mUmGmGmAmUmAmGmUmUmGmGmUmGmGmGmAmGmAmGmGmAmGmUmGmGmGmUmG/3ROX_N/ | '''IDT DNA Oligo Order:''' mUmGmGmAmUmAmGmUmUmGmGmUmGmGmGmAmGmAmGmGmAmGmUmGmGmGmUmG/3ROX_N/ | ||
| − | This is an improved version of [[Part:BBa_K779116]], with this design addressing the low melting temperature, orthogonality and small length of the previous iteration. | + | This is an improved version of [[Part:BBa_K779116]], with this design addressing the low melting temperature, orthogonality and small length of the previous iteration. Refer to '''[[Part:BBa_K779116]]''' for data on the ROX fluorophore. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 19:12, 7 October 2012
Long reporter bottom strand MammoBlock
Long reporter bottom strand to bind to Part:BBa_K779501 and Part:BBa_K779500.
Modifications: 3' ROX florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
IDT DNA Oligo Order: mUmGmGmAmUmAmGmUmUmGmGmUmGmGmGmAmGmAmGmGmAmGmUmGmGmGmUmG/3ROX_N/
This is an improved version of Part:BBa_K779116, with this design addressing the low melting temperature, orthogonality and small length of the previous iteration. Refer to Part:BBa_K779116 for data on the ROX fluorophore.
Usage and Biology
Provides a quenchable fluorophore. When annealed to a top strand (see parts above), the ROX fluorophore is quenched by an Iowa Black RQ quencher. When an input strand (Part:BBa_K779503) is added, strand displacement occurs and the quencher top strand is released.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]