Difference between revisions of "Part:BBa K783055"

Latest revision as of 18:53, 7 October 2012

Level 0 MoClo Destination Vector with AB

This is a Level 0 MoClo destination vector with flanking sites A on the 5' side and site B on the 3' side of the alpha fragment of lacZ.

The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 251
Illegal XbaI site found at 278
Illegal SpeI site found at 1
Illegal SpeI site found at 7
Illegal SpeI site found at 535
Illegal PstI site found at 290
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 251
Illegal SpeI site found at 1
Illegal SpeI site found at 7
Illegal SpeI site found at 535
Illegal PstI site found at 290
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 251
Illegal BamHI site found at 272
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 251
Illegal XbaI site found at 278
Illegal SpeI site found at 1
Illegal SpeI site found at 7
Illegal SpeI site found at 535
Illegal PstI site found at 290
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 251
Illegal XbaI site found at 278
Illegal SpeI site found at 1
Illegal SpeI site found at 7
Illegal SpeI site found at 535
Illegal PstI site found at 290
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 14
Illegal BsaI.rc site found at 528

@@ Line 4: / Line 4: @@
 This is a Level 0 MoClo destination vector with flanking sites A on the 5' side and site B on the 3' side of the alpha fragment of lacZ.
-The fusion site letters refer to 4bp fusion sites:
+The fusion site letters refer to 4bp fusion sites: A	= GGAG;	B	= TACT; C	= AATG; D	= AGGT;	E	= GCTT; 	F	= CGCT; 	G	= TGCC; H	= ACTA.
-A	= GGAG;	E	= GCTT;
-B	= TACT;	F	= CGCT;
-C	= AATG;	G	= TGCC;
-D	= AGGT;	H	= ACTA.