Difference between revisions of "Part:BBa K783052"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K783052 short</partinfo> | <partinfo>BBa_K783052 short</partinfo> | ||
| − | This is a MoClo converted version of BBa_C0051, which is the coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051). It has two MoClo fusion sites flanking it. | + | This is a MoClo converted version of BBa_C0051, which is the coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051). |
| + | |||
| + | It has two MoClo fusion sites flanking it: 5' C [part] D 3' | ||
| + | |||
| + | The fusion site letters refer to 4bp fusion sites: A = GGAG; E = GCTT; B = TACT; F = CGCT; C = AATG; G = TGCC; D = AGGT; H = ACTA. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 18:49, 7 October 2012
This is a MoClo converted version of BBa_C0051
This is a MoClo converted version of BBa_C0051, which is the coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
It has two MoClo fusion sites flanking it: 5' C [part] D 3'
The fusion site letters refer to 4bp fusion sites: A = GGAG; E = GCTT; B = TACT; F = CGCT; C = AATG; G = TGCC; D = AGGT; H = ACTA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]