Difference between revisions of "Part:BBa K783082"
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pMmoR is a promoter isolated from Methylosinus trichosporium OB3b and is in the sigma-54 regulatory family of promoters. The gene mmoR from Methylosinus trichosporium OB3b induces pMmoR and copper represses that activity. | pMmoR is a promoter isolated from Methylosinus trichosporium OB3b and is in the sigma-54 regulatory family of promoters. The gene mmoR from Methylosinus trichosporium OB3b induces pMmoR and copper represses that activity. | ||
| − | This has two MoClo fusion sites flanking the part. | + | This has two MoClo fusion sites flanking the part: 5' C [part] D 3' |
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| + | The fusion site letters refer to 4bp fusion sites: A = GGAG; E = GCTT; B = TACT; F = CGCT; C = AATG; G = TGCC; D = AGGT; H = ACTA. | ||
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Revision as of 18:47, 7 October 2012
pMmoR, a sigma 54-regulated promoter MoClo format
pMmoR is a promoter isolated from Methylosinus trichosporium OB3b and is in the sigma-54 regulatory family of promoters. The gene mmoR from Methylosinus trichosporium OB3b induces pMmoR and copper represses that activity.
This has two MoClo fusion sites flanking the part: 5' C [part] D 3'
The fusion site letters refer to 4bp fusion sites: A = GGAG; E = GCTT; B = TACT; F = CGCT; C = AATG; G = TGCC; D = AGGT; H = ACTA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]