Difference between revisions of "Part:BBa K935002:Experience"

Revision as of 14:54, 6 October 2012

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Response of BBa_935002 in E.coli.

A dilution series of sodium arsenate was created in 100 uL of LB broth in 96-well tissue culture plates. Replicate 100ul of cells were plated at a density of 2.5*10^8 cells per mL and incubated for 12 hrs at 37C. Epifluoresence (Ex518/Em607) was measured on a Molecular Devices FilterMax F3 reader (PMT 2 sec) and divided by the cell number determined by an A600 scan of the same wells to derive the flourescence per cell (which was multiplied by 10^4 for charting convenience). Error bars represent standard deviation of the sample mean.

User Reviews

UNIQ4fb95cf3f3ba6100-partinfo-00000000-QINU UNIQ4fb95cf3f3ba6100-partinfo-00000001-QINU

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 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 how you used this part and how it worked out.
-===Applications of BBa_K935002===
+'''Response of BBa_935002 in E.coli.'''
+A dilution series of sodium arsenate was created in 100 uL of LB broth in 96-well tissue culture plates.  Replicate 100ul of cells were plated at a density of 2.5*10^8 cells per mL  and incubated for 12 hrs at 37C.  Epifluoresence (Ex518/Em607) was measured on a Molecular Devices FilterMax F3 reader (PMT 2 sec) and divided by the cell number determined by an A600 scan of the same wells to derive the flourescence per cell (which was multiplied by 10^4 for charting convenience). Error bars represent standard deviation of the sample mean.
 ===User Reviews===