Difference between revisions of "Part:BBa K769003:Design"
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===Design Notes=== | ===Design Notes=== | ||
[http://2012.igem.org/Team:Tokyo-NoKoGen/Project/rhodopsin] | [http://2012.igem.org/Team:Tokyo-NoKoGen/Project/rhodopsin] | ||
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| + | https://static.igem.org/mediawiki/2012/thumb/c/cb/Rhodopsin2.jpg/800px-Rhodopsin2.jpg | ||
===Source=== | ===Source=== | ||
Revision as of 07:28, 6 October 2012
Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator-PompR-RBS-GFP-Double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2626
Illegal XhoI site found at 2527 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 134
Illegal NgoMIV site found at 392 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1088
Illegal SapI site found at 862
Design Notes
[http://2012.igem.org/Team:Tokyo-NoKoGen/Project/rhodopsin]
Source
Halobacteria