Difference between revisions of "Part:BBa K778010"
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FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] . | FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] . | ||
E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). | E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). | ||
| + | fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from [[Part:BBa K778001|wild-type fhlA]]. | ||
| + | This mutation increased H<sub>2</sub> production under the conditions in the previous research (Viviana et.al.2009). | ||
| + | Actually, in our research (team UT-Tokyo 2012<!--[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]-->), this mutation increased H<sub>2</sub> production as the following figure.<!--this mutation did not increase H2 production.--> (The full construct in this experiment is [[Part:BBa K778008]]) | ||
| − | Detailed Information:[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli | + | [[Image:UT Tokyo H2 1001+eg.png]] |
| + | <!--[[Image:UT Tokyo H2 1001.png]]--> | ||
| + | |||
| + | Detailed Information :Our wiki (UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] | ||
| + | The results shown on the wiki are different from the latest result on this parts.igem page. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 06:36, 6 October 2012
hycAp-GFPgenerator-plac-FhlAgenerator
You can "enhance" and "see" H2 production with this device.
hycAp-GFPgenerator-plac-FhlAgenerator
GFP is a reportor of H2 production (strictly speaking, reporter of hycAp activation Part:BBa K778000 (hycAp is the promotor of formate hydrogen lyase of genome)).
FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from wild-type fhlA. This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009). Actually, in our research (team UT-Tokyo 2012), this mutation increased H2 production as the following figure. (The full construct in this experiment is Part:BBa K778008)
Detailed Information :Our wiki (UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] The results shown on the wiki are different from the latest result on this parts.igem page.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3266
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2545
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 828
