Difference between revisions of "Part:BBa K778005"

Revision as of 05:49, 6 October 2012

RBS-fhlA363-d.term

FhlA-E363G generator without promoter (RBS-fhlAE363G-d.term)

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from wild-type fhlA. This mutation increased H₂ production under the conditions in the previous research (Viviana et.al.2009). Actually, in our research (team UT-Tokyo 2012), this mutation increased H₂ production as the following figure. (The full construct in this experiment is Part:BBa K778008)

Detailed Information :Our wiki (UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]The results shown on the wiki is diffaerent from the latest result on this page.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2115
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1394
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] .
 E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
-fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).
+fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from [[Part:BBa K778001|wild-type fhlA]].
-This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009).
+This mutation increased H<sub>2</sub>  production under the conditions in the previous research (Viviana et.al.2009).
-However, in our  research (team UT-Tokyo 2012[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]), this mutation did not increase H2 production.
+Actually, in our research (team UT-Tokyo 2012<!--[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]-->), this mutation increased H<sub>2</sub> production as the following figure.<!--this mutation did not increase H2 production.--> (The full construct in this experiment is [[Part:BBa K778008]])
-Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]
+[[Image:UT Tokyo H2 1001+eg.png]]
+<!--[[Image:UT Tokyo H2 1001.png]]-->
+Detailed Information :Our wiki (UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]The results shown on the wiki is diffaerent from the latest result on this page.
 <!-- Add more about the biology of this part here