Difference between revisions of "Part:BBa K811000"

Revision as of 01:41, 6 October 2012

Cytolysin A (ClyA)

Cytolysin A (ClyA) pore forming protein causes cell lysis. After cloning into expression vector, clyA expression and activity can be visualized on 5% Sheep's Blood Agar plates. Expected transcript size: 34kDa

Cytolysin A (ClyA)

Cytolysin A (ClyA) pore forming protein causes cell lysis. After cloning into expression vector, clyA expression and activity can be visualized on 5% Sheep's Blood Agar plates. Expected transcript size: 34kDa

Usage and Biology

ClyA is a protein native to E. coli, Shigella flexneri, and Salmonella typhi that is capable of forming 13-mer pore complexes in a redox-independent manner. Expression of clyA in the absence of other hemolytic toxins is sufficient to induce hemolysis experimentally, and is therefore considered to be a potent cytolytic agent. Unlike a similar protein, HlyA, ClyA is not synthesized as a protoxin, which requires further posttranslational modifications to become active. ClyA functional immediately following translation of mRNA to protein. In vitro and i n vivo,

Sequence and Features