Difference between revisions of "Part:BBa K811000"
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<partinfo>BBa_K811000 short</partinfo> | <partinfo>BBa_K811000 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | ClyA is a protein native to E. coli, Shigella flexneri, and Salmonella typhi that is capable of forming 13-mer pore complexes in a redox-independent manner. Expression of clyA in the absence of other hemolytic toxins is sufficient to induce hemolysis experimentally, and is therefore considered to be a potent cytolytic agent. | |
| + | Unlike a similar protein, HlyA, ClyA is not synthesized as a protoxin, which requires further posttranslational modifications to become active. ClyA functional immediately following translation of mRNA to protein. In vitro and in vivo, | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 00:43, 6 October 2012
Cytolysin A (ClyA)
Cytolysin A (ClyA) pore forming protein causes cell lysis. After cloning into expression vector, clyA expression and activity can be visualized on 5% Sheep's Blood Agar plates. Expected transcript size: 34kDa
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]