Difference between revisions of "Part:BBa K769003"
 
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<partinfo>BBa_K769003 short</partinfo>
 
<partinfo>BBa_K769003 short</partinfo>
  
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Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator-PompR-RBS-GFP-Double terminator
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This device is constructed with BBa_K769000 and BBa_K769001. When SRII receives blue light, EnvZ maybe  phosphorylate OmpR. Phosphorylated OmpR activates OmpC promotor, GFP is expressed.   
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<BR>https://static.igem.org/mediawiki/parts/8/84/Safety.png
 
<BR>https://static.igem.org/mediawiki/parts/8/84/Safety.png
  

Latest revision as of 04:45, 5 October 2012

Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator-PompR-RBS-GFP-Double terminator

This device is constructed with BBa_K769000 and BBa_K769001. When SRII receives blue light, EnvZ maybe phosphorylate OmpR. Phosphorylated OmpR activates OmpC promotor, GFP is expressed.


Safety.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2626
    Illegal XhoI site found at 2527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 134
    Illegal NgoMIV site found at 392
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1088
    Illegal SapI site found at 862