Difference between revisions of "Part:BBa K769004"
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<partinfo>BBa_K769004 short</partinfo> | <partinfo>BBa_K769004 short</partinfo> | ||
− | + | This chimeric fusion protein is blue light sensor. This protein is constructed with sensor domain and kinase domain. Sensor domain is Natronomonas pharaonis sensory rhodopsin II (SRII) fused by a flexible linker to halobacterium transducer protein II (HtrII). SRII is a repellent receptor sensing blue light (λ max 487 nm). SRII interacts with HtrII, which in turn controls the activity of a histidine kinase. Histidine kinase we used is the kinase domain of EnvZ. EnvZ phosphorylates response regulator of two-compoment system, OmpR. Phosphorylated OmpR activates OmpC promoter. | |
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<BR>https://static.igem.org/mediawiki/parts/8/84/Safety.png | <BR>https://static.igem.org/mediawiki/parts/8/84/Safety.png | ||
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Revision as of 02:41, 5 October 2012
NpSRII-9a.a.linker_NpHtrII-EnvZ
This chimeric fusion protein is blue light sensor. This protein is constructed with sensor domain and kinase domain. Sensor domain is Natronomonas pharaonis sensory rhodopsin II (SRII) fused by a flexible linker to halobacterium transducer protein II (HtrII). SRII is a repellent receptor sensing blue light (λ max 487 nm). SRII interacts with HtrII, which in turn controls the activity of a histidine kinase. Histidine kinase we used is the kinase domain of EnvZ. EnvZ phosphorylates response regulator of two-compoment system, OmpR. Phosphorylated OmpR activates OmpC promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 73
Illegal NgoMIV site found at 331 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1027
Illegal SapI site found at 801