Difference between revisions of "Part:BBa C0012"
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Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bbpart>BBa_R0010</bbpart> and PLlac01 hybrid regulator <bbpart>BBa_R0011</bbpart> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. | Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bbpart>BBa_R0010</bbpart> and PLlac01 hybrid regulator <bbpart>BBa_R0011</bbpart> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. | ||
| − | A rapid | + | A rapid degradation tail (LVA) has been added to improve the High to Low performance of this part. |
[[Image:LacRep-black.jpg]] | [[Image:LacRep-black.jpg]] | ||
Revision as of 08:28, 12 June 2006
lacI repressor from E. coli (+LVA)
Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.
A rapid degradation tail (LVA) has been added to improve the High to Low performance of this part.
