Difference between revisions of "Part:BBa K804013:Experience"
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===MetA Knockout Complementation by British Columbia iGEM 2012=== | ===MetA Knockout Complementation by British Columbia iGEM 2012=== | ||
| − | This part consists of an | + | This part consists of an rhamnose promoter, a strong RBS, and the MetA coding gene. It is unable to complement a MetA knockout, and the growth rate appears to unaffected by the amount of rhamnose that is added. The data for the Rhamnose Inducible TrpA Coding Gene is also shown on this graph. |
https://static.igem.org/mediawiki/parts/thumb/d/d2/Rhamnose_part_test.png/800px-Rhamnose_part_test.png | https://static.igem.org/mediawiki/parts/thumb/d/d2/Rhamnose_part_test.png/800px-Rhamnose_part_test.png | ||
Latest revision as of 04:03, 4 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K804013
User Reviews
UNIQb71741e848552737-partinfo-00000000-QINU UNIQb71741e848552737-partinfo-00000001-QINU
MetA Knockout Complementation by British Columbia iGEM 2012
This part consists of an rhamnose promoter, a strong RBS, and the MetA coding gene. It is unable to complement a MetA knockout, and the growth rate appears to unaffected by the amount of rhamnose that is added. The data for the Rhamnose Inducible TrpA Coding Gene is also shown on this graph.
To generate this graph we cultured an E. coli MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of rhamnose, and measured the OD600 every 15 minutes.