Difference between revisions of "Part:BBa K804013:Experience"
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| + | ===MetA Knockout Complementation by British Columbia iGEM 2012=== | ||
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| + | This part consists of an arabinose promoter, a strong RBS, and the MetA coding gene. It is unable to complement a MetA knockout, and the growth rate appears to unaffected by the amount of arabinose that is added. The data for the Rhamnose Inducible TrpA Coding Gene is also shown on this graph. | ||
| + | https://static.igem.org/mediawiki/parts/thumb/d/d2/Rhamnose_part_test.png/800px-Rhamnose_part_test.png | ||
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| + | To generate this graph we cultured an E. coli MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of rhamnose, and measured the OD600 every 15 minutes. | ||
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| + | - [[User:jacobtoth|Jacob Toth]] | ||
Revision as of 03:51, 4 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K804013
User Reviews
UNIQe1d8b846c873ecf4-partinfo-00000000-QINU UNIQe1d8b846c873ecf4-partinfo-00000001-QINU
MetA Knockout Complementation by British Columbia iGEM 2012
This part consists of an arabinose promoter, a strong RBS, and the MetA coding gene. It is unable to complement a MetA knockout, and the growth rate appears to unaffected by the amount of arabinose that is added. The data for the Rhamnose Inducible TrpA Coding Gene is also shown on this graph.
To generate this graph we cultured an E. coli MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of rhamnose, and measured the OD600 every 15 minutes.