Difference between revisions of "Part:BBa K902053"
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<partinfo>BBa_K902053 short</partinfo> | <partinfo>BBa_K902053 short</partinfo> | ||
| − | <html><p><a href="https://parts.igem.org/Part:BBa_K902052">DszB</a> under a constitutive TetR promoter (<a href="https://parts.igem.org/ | + | <html><p><a href="https://parts.igem.org/Part:BBa_K902052">DszB</a> under a constitutive TetR promoter (<a href="https://parts.igem.org/Part:BBa_J13002">BBa_J13002</a>)</html> |
<html><p> | <html><p> | ||
Latest revision as of 03:01, 4 October 2012
Constitutive DszB generator (J13002-DszB)
DszB under a constitutive TetR promoter (BBa_J13002)
This sequence codes for HBPS desulfinase (DszB), which is a monomeric enzyme that carries out the last step of the 4S desulfurization pathway. This step carries out the conversion of 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl, releasing the sulfur in the form of sulfite. Naturally, this enzyme has the lowest activity of all the desulfurization genes involved in the 4S pathway, and thus this step is the rate limiting step. Because of this, the sequence has been mutated Y63F to increase enzyme activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 506
Illegal XhoI site found at 498 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 213
- 1000COMPATIBLE WITH RFC[1000]