Difference between revisions of "Part:BBa K781006"
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This part can be used as a template for making other PCR overlap insertions. After transforming the product, and digesting the template plasmid with Dpn1, any template that has not been successfully digested will form red colonies. | This part can be used as a template for making other PCR overlap insertions. After transforming the product, and digesting the template plasmid with Dpn1, any template that has not been successfully digested will form red colonies. | ||
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| + | Please refer to the page for K781001 for characterization results until this page is updated. | ||
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Revision as of 02:53, 4 October 2012
[R0010][B0034] - RFP-FliC Deletion Chimera
This is the modified RFP protein obtained from J04450, that has been incorporated into the E. coli flagellin as a deletion.
This part can be used as a template for making other PCR overlap insertions. After transforming the product, and digesting the template plasmid with Dpn1, any template that has not been successfully digested will form red colonies.
Please refer to the page for K781001 for characterization results until this page is updated.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1884
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 529
Illegal AgeI site found at 1362
Illegal AgeI site found at 1474 - 1000COMPATIBLE WITH RFC[1000]