Difference between revisions of "Part:BBa K902058"
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<a href="https://parts.igem.org/Part:BBa_K902057"><i>hpaC</i></a> under control of the lacI promotor (<a href="https://parts.igem.org/Part:BBa_J04500">BBa_J04500</a>). </p></html>
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This composite part contains the gene encoding <I>E. coli's</i><a href="https://parts.igem.org/Part:BBa_K902057"></a><i>hpaC</i> enzyme under control of the lacI promotor (<a href="https://parts.igem.org/Part:BBa_J04500">BBa_J04500</a>). HpaC is an oxidoreductase capable of recycling FMN to FMNH<sub>2</sub> while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.</p><br>
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</html>[[Image:File-Ucalgary team sulfur 4s enzyme pathway diagram.png|centre]]<html></html> </p></html>
  
 
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Revision as of 02:42, 4 October 2012

hpaC inducible generator with lacI promotor (J04500)

This composite part contains the gene encoding E. coli'shpaC enzyme under control of the lacI promotor (BBa_J04500). HpaC is an oxidoreductase capable of recycling FMN to FMNH2 while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.


File-Ucalgary team sulfur 4s enzyme pathway diagram.png
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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 509