Difference between revisions of "Part:BBa K902052:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K902052 short</partinfo> | <partinfo>BBa_K902052 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | Amplified from a plasmid in an environmental isolate of Rhodococcus shown to be an active desulfurizer. | + | Amplified from a plasmid in an environmental isolate of <i>Rhodococcus</i> shown to be an active desulfurizer. |
===References=== | ===References=== | ||
Revision as of 02:40, 4 October 2012
HBPS desulfinase (DszB) from R. erythropolis (mutated for improved activity)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 426
Illegal XhoI site found at 418 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 133
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mutations of internal PstI and NotI cut sites in order to make biobrick compatible, as well as an added mutation of Y63F in the protein to increase its activity.
Source
Amplified from a plasmid in an environmental isolate of Rhodococcus shown to be an active desulfurizer.