Difference between revisions of "Part:BBa K779501"
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When annealed to [[Part:BBa K779502]] acts as a reporter for strand displacement reactions. | When annealed to [[Part:BBa K779502]] acts as a reporter for strand displacement reactions. | ||
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===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 02:35, 4 October 2012
Long reporter top strand MammoBlock
Long reporter top strand to be annealed with Part:BBa K779502.
Modifications: 5' Iowa Black RQ quencher, 3' Alexa 488 florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
IDT DNA oligo order: /5IAbRQ/mCmAmCmCmCmAmCmUmCmCmUmCmUmCmCmCmAmCmCmA/3AlexF488N/
Usage and Biology
When annealed to Part:BBa K779502 acts as a reporter for strand displacement reactions.
<img src="
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Sequence and Features
Sequence is designed to be orthogonal to the HEK293 transcriptome (see Design). Design is improved on the part Part:BBa_K779121, with goals being a higher melting temperature, more orthogonal and longer domain.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]