Difference between revisions of "Part:BBa K902007:Design"
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===References=== | ===References=== | ||
+ | Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S.The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.Microbiology. 1996 Jul;142 ( Pt 7):1659-65. |
Latest revision as of 02:32, 4 October 2012
Beta-D-glucoside glucohydrolase (bglX) with strong RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1639
Illegal AgeI site found at 1861
Illegal AgeI site found at 2050 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
An illegal pstI site was removed from the 15th and 16th amino acid positions via site directed mutagenesis.
Source
The beta-D-glucoside glucohydrolase (bglX) gene was amplified from TOP10 E. coli via polymerase chain reaction.
References
Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S.The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.Microbiology. 1996 Jul;142 ( Pt 7):1659-65.