Difference between revisions of "Part:BBa K902006:Design"

 
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===References===
 
===References===
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Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S.The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.Microbiology. 1996 Jul;142 ( Pt 7):1659-65

Latest revision as of 02:31, 4 October 2012

Beta-D-glucoside glucohydrolase (bglX) inducible generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1847
    Illegal AgeI site found at 2069
    Illegal AgeI site found at 2258
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An illegal pstI site was removed from the 15th and 16th amino acid positions via site-directed mutagenesis.


Source

The beta-D-glucoside glucohydrolase (bglX) gene was amplified from the genome of TOP10 E. coli via polymerase chain reaction.

References

Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S.The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.Microbiology. 1996 Jul;142 ( Pt 7):1659-65