Difference between revisions of "Part:BBa K902004:Design"

 
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===References===
 
===References===
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Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S. The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.Microbiology. 1996 Jul;142 ( Pt 7):1659-65.

Revision as of 02:26, 4 October 2012

Periplasmic beta-D-glucoside glucohydrolase (bglX)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1621
    Illegal AgeI site found at 1843
    Illegal AgeI site found at 2032
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An illegal pstI site at the 15th and 16th amino acid position was mutated out via site-directed mutagenesis.


Source

This gene was amplified from the genome of E. coli via polymerase chain reaction.

References

Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S. The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.Microbiology. 1996 Jul;142 ( Pt 7):1659-65.