Difference between revisions of "Part:BBa K936033"
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| − | Mutant of the Polyetheylene Terephtalate degrading protein LC Cutinase (Bba_K936000). The phenylalanine at the 125th amino acid position is changed to a tyrosine. This part was created using site directed mutagenesis to change a "TTC" codon to a "TAT" codon. | + | Mutant of the Polyetheylene Terephtalate degrading protein LC Cutinase (Bba_K936000). The phenylalanine at the 125th amino acid position is changed to a tyrosine. This part was created using site directed mutagenesis to change a "TTC" codon to a "TAT" codon. <br> |
| + | https://static.igem.org/mediawiki/2012/a/aa/UC_Davis_First_pNPB_Run.png <br>(Above Graph) Results of pNPB assay detailing the constitutive construct as having the highest esterase activity.https://static.igem.org/mediawiki/2012/6/62/UC_Davis_Third_pNPB_Run.png<br>(Above Graphs) The graphs above detail higher activity among the constitutive construct and some mutant variants in esterase activity compared to the control and wild type per unit cell. <br>https://static.igem.org/mediawiki/2012/5/59/UC_Davis_Last_pNPB_Run.png<br> (Above graph) This graph represents a possible production of cutinase by the cells upon entering stationary phase.<br><br> | ||
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Latest revision as of 02:26, 4 October 2012
LC Cutinase mutant F125Y
Mutant of the Polyetheylene Terephtalate degrading protein LC Cutinase (Bba_K936000). The phenylalanine at the 125th amino acid position is changed to a tyrosine. This part was created using site directed mutagenesis to change a "TTC" codon to a "TAT" codon.
(Above Graph) Results of pNPB assay detailing the constitutive construct as having the highest esterase activity.
(Above Graphs) The graphs above detail higher activity among the constitutive construct and some mutant variants in esterase activity compared to the control and wild type per unit cell. 
(Above graph) This graph represents a possible production of cutinase by the cells upon entering stationary phase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 600
- 1000COMPATIBLE WITH RFC[1000]