Difference between revisions of "Part:BBa K936021"
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| − | LC Cutinase coding sequence with polyhistidine tag attached. The polyhistidine tag allows our research team to perform assays to discover where our enzyme is being released, both in the media and in the cell. | + | LC Cutinase coding sequence with polyhistidine tag attached. The polyhistidine tag allows our research team to perform assays to discover where our enzyme is being released, both in the media and in the cell. <br> |
| − | + | https://static.igem.org/mediawiki/2012/a/aa/UC_Davis_First_pNPB_Run.png <br>(Above Graph) Results of pNPB assay detailing the constitutive construct as having the highest esterase activity.https://static.igem.org/mediawiki/2012/6/62/UC_Davis_Third_pNPB_Run.png<br>(Above Graphs) The graphs above detail higher activity among the constitutive construct and some mutant variants in esterase activity compared to the control and wild type per unit cell. <br>https://static.igem.org/mediawiki/2012/5/59/UC_Davis_Last_pNPB_Run.png<br> (Above graph) This graph represents a possible production of cutinase by the cells upon entering stationary phase.<br><br> | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 02:23, 4 October 2012
Cutinase and polyhistidine tag
LC Cutinase coding sequence with polyhistidine tag attached. The polyhistidine tag allows our research team to perform assays to discover where our enzyme is being released, both in the media and in the cell.
(Above Graph) Results of pNPB assay detailing the constitutive construct as having the highest esterase activity.
(Above Graphs) The graphs above detail higher activity among the constitutive construct and some mutant variants in esterase activity compared to the control and wild type per unit cell. 
(Above graph) This graph represents a possible production of cutinase by the cells upon entering stationary phase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 600
- 1000COMPATIBLE WITH RFC[1000]