Difference between revisions of "Part:BBa K902024:Design"
(→Source) |
(→Design Notes) |
||
Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | There are no illegal cut sites in the moaABCDE operon. | |
− | + | ||
− | + | ||
===Source=== | ===Source=== |
Latest revision as of 02:15, 4 October 2012
moa Operon (MoaABCDE)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 539
Illegal BamHI site found at 665 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 952
Illegal AgeI site found at 1851 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 603
Illegal SapI.rc site found at 1964
Design Notes
There are no illegal cut sites in the moaABCDE operon.
Source
The moaABCDE operon was first seen to be used in conjunction with the Moco riboswitch in the paper cited in the reference sequence. The sequence was derived from Profiling of E.Coli Chromosome (PEC) and NCBI Gene Database.
References
1) Regulski EE, Moy RH, Weinberg Z, Barrick JE, Yao Z, Ruzzo WL, Breaker RR. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.Mol Microbiol. 2008 May; 68(4): 918–932