Difference between revisions of "Part:BBa K900000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The gene was codon optimized for yeast and internal restriction sites were removed for | + | The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with our Golden Gate cloning scheme. |
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===Source=== | ===Source=== | ||
Revision as of 01:35, 4 October 2012
Design Notes
The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with our Golden Gate cloning scheme.
Source
Synthesized in house and cloned into addgene plasmid [http://www.addgene.org/11911/ l11911] Previously engineered version of RFP from Subach et al., 2009.
References
Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009).