Difference between revisions of "Part:BBa K844010"

Revision as of 01:27, 4 October 2012

Enhanced tRNA Promoter for ''E. coli''

Usage and Biology

This tRNA terminator seqeuence is based around the downstream sequence of the E. coli Ala(GCC) tRNA. It contains the region of 3-6 T’s that is characteristic of tRNA terminators and an additional 10 bp of native sequence. This additional region of native sequence is to ensure RNA termination and to act as a small spacer region between tRNA genes.

It is important to use a tRNA terminator when expressing tRNA parts, as rho-dependent and rho-independent promoters rely on additional RNA sequence after the tRNA gene to function, and the addition of more RNA bases on a tRNA can severely affect its ability to function.

Related Parts

BBa_K8440011 - This part is a tRNA specific terminator for E. coli

References

Bauer B.F., Elford R.M., and Holmes W.M. 1993. Mutagenesis and functional analysis of the Escherichia coli tRNA (1Leu) promoter. Mol microbio. 7:265–273.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 ===Usage and Biology===
-This part is a mutated version of the ''E. coli'' leuV tRNA promoter. This part is based on the ZH14 sequence from Bauer et al. (1993). This promoter has approximately normal growth-rate dependent regulation but 12.0x normal promoter strength in rich medium.
+This tRNA terminator seqeuence is based around the downstream sequence of the E. coli Ala(GCC) tRNA. It contains the region of 3-6 T’s that is characteristic of tRNA terminators and an additional 10 bp of native sequence. This additional region of native sequence is to ensure RNA termination and to act as a small spacer region between tRNA genes.
-It is important to use a tRNA promoter to express tRNA parts, as other promoters will recruit RNA polymerase II, which only synthesizes mRNA. The tRNA promoter will recruit RNA polymerase III, which is the correct polymerase for synthesizing tRNAs.
+It is important to use a tRNA terminator when expressing tRNA parts, as rho-dependent and rho-independent promoters rely on additional RNA sequence after the tRNA gene to function, and the addition of more RNA bases on a tRNA can severely affect its ability to function.