Difference between revisions of "Part:BBa K902062"
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| − | CAR (carboxylic acid reductase) is a gene present in <i>Nocardia iowensis</i> that is an ATP/NADPH-dependent carboxylic acid reductase. It is highly non-specific and able to reduce a range of carboxylic acids. | + | <html>CAR (carboxylic acid reductase) is a gene present in <i>Nocardia iowensis</i> that is an ATP/NADPH-dependent carboxylic acid reductase. It is highly non-specific and able to reduce a range of carboxylic acids. It is dependent on <a href="https://parts.igem.org/Part:BBa_K902061"><i>NPT</i></a> for full function.</html> |
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| + | [[Image:Ucalgary Decarboxylation Team CAR Mechanism.jpg|400px|center]] | ||
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Revision as of 01:26, 4 October 2012
CAR (Nocardia carboxylic acid reductase)
CAR (carboxylic acid reductase) is a gene present in Nocardia iowensis that is an ATP/NADPH-dependent carboxylic acid reductase. It is highly non-specific and able to reduce a range of carboxylic acids. It is dependent on NPT for full function.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1302
Illegal NotI site found at 1691
Illegal NotI site found at 2452 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1016
Illegal BglII site found at 1448
Illegal BglII site found at 1619
Illegal BglII site found at 2102
Illegal XhoI site found at 1498
Illegal XhoI site found at 1582
Illegal XhoI site found at 2509 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3108
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 256
Illegal BsaI.rc site found at 1402
Illegal BsaI.rc site found at 1798