Difference between revisions of "Part:BBa K844010"
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<partinfo>BBa_K844010 short</partinfo> | <partinfo>BBa_K844010 short</partinfo> | ||
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| + | ===Usage and Biology=== | ||
This part is a mutated version of the ''E. coli'' leuV tRNA promoter. This part is based on the ZH14 sequence from Bauer et al. (1993). This promoter has approximately normal growth-rate dependent regulation but 12.0x normal promoter strength in rich medium. | This part is a mutated version of the ''E. coli'' leuV tRNA promoter. This part is based on the ZH14 sequence from Bauer et al. (1993). This promoter has approximately normal growth-rate dependent regulation but 12.0x normal promoter strength in rich medium. | ||
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It is important to use a tRNA promoter to express tRNA parts, as other promoters will recruit RNA polymerase II, which only synthesizes mRNA. The tRNA promoter will recruit RNA polymerase III, which is the correct polymerase for synthesizing tRNAs. | It is important to use a tRNA promoter to express tRNA parts, as other promoters will recruit RNA polymerase II, which only synthesizes mRNA. The tRNA promoter will recruit RNA polymerase III, which is the correct polymerase for synthesizing tRNAs. | ||
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| − | === | + | ===Related Parts=== |
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| + | [https://parts.igem.org/Part:BBa_K844011 BBa_K8440011] - This part is a tRNA specific terminator for ''E. coli'' | ||
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| + | ===References=== | ||
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| + | Bauer B.F., Elford R.M., and Holmes W.M. 1993. Mutagenesis and functional analysis of the Escherichia coli tRNA (1Leu) promoter. Mol microbio. 7:265–273. | ||
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Revision as of 01:25, 4 October 2012
Enhanced tRNA Promoter for ''E. coli''
Usage and Biology
This part is a mutated version of the E. coli leuV tRNA promoter. This part is based on the ZH14 sequence from Bauer et al. (1993). This promoter has approximately normal growth-rate dependent regulation but 12.0x normal promoter strength in rich medium.
It is important to use a tRNA promoter to express tRNA parts, as other promoters will recruit RNA polymerase II, which only synthesizes mRNA. The tRNA promoter will recruit RNA polymerase III, which is the correct polymerase for synthesizing tRNAs.
Related Parts
BBa_K8440011 - This part is a tRNA specific terminator for E. coli
References
Bauer B.F., Elford R.M., and Holmes W.M. 1993. Mutagenesis and functional analysis of the Escherichia coli tRNA (1Leu) promoter. Mol microbio. 7:265–273.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]