Difference between revisions of "Part:BBa K847041:Design"

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===References===
 
===References===
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Chattopadhyay, M. K. (2006). Mechanism of bacterial adaptation to low temperature. ''J. Biosci., 31'' (1), 157-165.
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Bonaterra, A., Camps, J., Montesinos, E. (2005). Osmotically induced trehalose and glycine betaine accumulation improves tolerance to desiccation, survival and efficacy of the postharvest biocontrol agent ''Pantoea agglomerans'' EPS125. ''FEMS Microbiol. Lett., 250'': 1-8.
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Cayley, S., Lewis, B. A., Record Jr., T. (1992). Origins of the osmoprotective properties of betaine and proline in ''Escherichia coli'' K-12. ''J. Bacteriol. 174''(5): 1586-1595.

Latest revision as of 01:24, 4 October 2012

''Escherichia coli'' betaine aldehyde dehydrogenase (betB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1160
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 322
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 73
    Illegal BsaI.rc site found at 631


Design Notes

This part was isolated via colony PCR from Escherichia coli. It does not contain promoter, RBS, transcriptional terminator, etc. These elements must be connected to this part for proper functionality.

Source

Escherichia coli

References

Chattopadhyay, M. K. (2006). Mechanism of bacterial adaptation to low temperature. J. Biosci., 31 (1), 157-165.

Bonaterra, A., Camps, J., Montesinos, E. (2005). Osmotically induced trehalose and glycine betaine accumulation improves tolerance to desiccation, survival and efficacy of the postharvest biocontrol agent Pantoea agglomerans EPS125. FEMS Microbiol. Lett., 250: 1-8.

Cayley, S., Lewis, B. A., Record Jr., T. (1992). Origins of the osmoprotective properties of betaine and proline in Escherichia coli K-12. J. Bacteriol. 174(5): 1586-1595.