Difference between revisions of "Part:BBa K902048:Experience"

Revision as of 01:22, 4 October 2012

XylE (J33204) was biobricked under the tetR promoter (R0040). This construct was confirmed by sequencing and used in visual assays. The visual assay shown below demonstrated that the XylE gene was expressed with the tetR promoter and that the resulting enzyme (catechol 2,3-dioxygenase) was functional. The colour change to bright yellow indicated the conversion of catechol to 2-hydroxymuconic semialdehyde.

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Fig.1 Cells transformed with the part BBa_K902048 were grown overnight in BHI media. The cells were spun down and washed in M9 minimal media. The tube on the left contains the supernatant without catechol. The tube on the right was brought to a catechol concentration of 0.1 M.

===Applications of BBa_K902048=== ===User Reviews=== BBa_K902048 StartReviews BBa_K902048 EndReviews

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 XylE (J33204) was biobricked under the tetR promoter (R0040).  This construct was confirmed by sequencing and used in visual assays.  The visual assay shown below demonstrated that the XylE gene was expressed with the tetR promoter and that the resulting enzyme (catechol 2,3-dioxygenase) was functional.  The colour change to bright yellow indicated the conversion of catechol to 2-hydroxymuconic semialdehyde.
-</html>[[image:UCalgary2012_Catechol_assay_1.jpg|500px|thumb||center]]<html>
-</html>[[image:UCalgary2012_Catechol_assay_%282%29.jpg|500px|thumb||center]]<html>
+</html>[[image:UCalgary2012_Catechol_assay_%282%29.jpg|500px|thumb|Fig.1 Cells transformed with the part BBa_K902048 were grown overnight in BHI media.  The cells were spun down and washed in M9 minimal media.  The tube on the left contains the supernatant without catechol.  The tube on the right was brought to a catechol concentration of 0.1 M.|center]]<html>
 ===Applications of BBa_K902048===