Difference between revisions of "Part:BBa K844010"

 
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<partinfo>BBa_K844010 short</partinfo>
 
<partinfo>BBa_K844010 short</partinfo>

Revision as of 01:22, 4 October 2012

Enhanced tRNA Promoter for ''E. coli''

This part is a mutated version of the E. coli leuV tRNA promoter. This part is based on the ZH14 sequence from Bauer et al. (1993). This promoter has approximately normal growth-rate dependent regulation but 12.0x normal promoter strength in rich medium.

It is important to use a tRNA promoter to express tRNA parts, as other promoters will recruit RNA polymerase II, which only synthesizes mRNA. The tRNA promoter will recruit RNA polymerase III, which is the correct polymerase for synthesizing tRNAs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]