Difference between revisions of "Part:BBa K902048:Experience"
| Line 2: | Line 2: | ||
XylE (J33204) was biobricked under the tetR promoter (R0040). This construct was confirmed by sequencing and used in visual assays. The visual assay shown below demonstrated that the XylE gene was expressed with the tetR promoter and that the resulting enzyme (catechol 2,3-dioxygenase) was functional. The colour change to bright yellow indicated the conversion of catechol to 2-hydroxymuconic semialdehyde. | XylE (J33204) was biobricked under the tetR promoter (R0040). This construct was confirmed by sequencing and used in visual assays. The visual assay shown below demonstrated that the XylE gene was expressed with the tetR promoter and that the resulting enzyme (catechol 2,3-dioxygenase) was functional. The colour change to bright yellow indicated the conversion of catechol to 2-hydroxymuconic semialdehyde. | ||
| − | </html>[[Image: | + | </html>[[Image:UCalgary2012_Catechol_assay_%282%29.jpg|400px|thumb||center]]<html> |
===Applications of BBa_K902048=== | ===Applications of BBa_K902048=== | ||
Revision as of 01:12, 4 October 2012
XylE (J33204) was biobricked under the tetR promoter (R0040). This construct was confirmed by sequencing and used in visual assays. The visual assay shown below demonstrated that the XylE gene was expressed with the tetR promoter and that the resulting enzyme (catechol 2,3-dioxygenase) was functional. The colour change to bright yellow indicated the conversion of catechol to 2-hydroxymuconic semialdehyde.
</html> ===Applications of BBa_K902048=== ===User Reviews===.jpg)