Difference between revisions of "Part:BBa J45007:Design"

(→‎Design Notes)
(→‎Design Notes)
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Designed Forward Strand (1 and 2):  
 
Designed Forward Strand (1 and 2):  
 
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG <b>ATG GAA GTT GTT GAA GTT C</b> -3'<br>
 
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG <b>ATG GAA GTT GTT GAA GTT C</b> -3'<br>
−
*LENGTH: 49<br>
+
*LENGTH: 19 <br>
−
*GC CONTENT: 46.9 %<br>
+
*GC CONTENT: 36.8 % <br>
−
*MELT TEMP: 67.6 ºC<br>
+
*MELT TEMP: 47.8 ºC <br>
 
<br>
 
<br>
−
Designed Reverse Strand (phBSMT2): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TA<b>T TAT TAA TTT ATT TTG GTC AAA C</b> -3'<br>
+
Designed Reverse Strand (phBSMT2): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT T<B>AA TTT ATT TTG GTC AAA GAG AC</b> -3'<br>
−
*LENGTH: 52<br>
+
*LENGTH: 22 <br>
−
*GC CONTENT: 38.5 %<Br>
+
*GC CONTENT: 27.3 % <br>
−
*MELT TEMP: 65.0 ºC<br>
+
*MELT TEMP: 47.1 ºC <br>
  
 
===Source===
 
===Source===

Revision as of 15:45, 9 June 2006


SAM:benzoic acid/salicylic acid carboxyl methyltransferase II; converts salicylic acid to methyl sal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204


Design Notes

Just CDS taken from GenBank

Designed Forward Strand (1 and 2): 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT C -3'

  • LENGTH: 19
  • GC CONTENT: 36.8 %
  • MELT TEMP: 47.8 ºC


Designed Reverse Strand (phBSMT2): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAA GAG AC -3'

  • LENGTH: 22
  • GC CONTENT: 27.3 %
  • MELT TEMP: 47.1 ºC

Source

Species: Petunia x hybrida from GenBank (AY233466); (also available: A. thaliana and N. suaveolens)

References

    • <biblio>Underwood05 pmid=15849311</biblio>
    • <biblio>Negre03 pmid=14630969</biblio>