Difference between revisions of "Part:BBa K847080:Experience"
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| − | + | '''Assay'''<BR> | |
| − | + | Liquid cultures of negative control, recA, dps, and sdaB transformed NEB5α E. coli were grown up over night at 37°C. After incubation, LB + amp media of pH 3.54, 4.60, 6.93, 9.07, 9.50 were created using HCl and NaOH. In a 96 well plate, 8 wells were filled with LB + amp blank. Next, 5uL of each transformed bacteria was placed in five different pH wells. This was replicated four times, thus, creating a 96 well plate with 8 blanks, and four replicates of each test construct at five different pH’s. Finally, the OD600 was measured every five minutes for 24 hours while the plate was incubated at 37°C, shook for 1 minute before every sample and between every sample in a spectrophotometer. <BR><BR> | |
| + | '''Results'''<BR> | ||
| + | Works. Refer to: http://2012.igem.org/Team:Stanford-Brown/HellCell/pH | ||
===Applications of BBa_K847080=== | ===Applications of BBa_K847080=== | ||
Revision as of 00:56, 4 October 2012
Assay
Liquid cultures of negative control, recA, dps, and sdaB transformed NEB5α E. coli were grown up over night at 37°C. After incubation, LB + amp media of pH 3.54, 4.60, 6.93, 9.07, 9.50 were created using HCl and NaOH. In a 96 well plate, 8 wells were filled with LB + amp blank. Next, 5uL of each transformed bacteria was placed in five different pH wells. This was replicated four times, thus, creating a 96 well plate with 8 blanks, and four replicates of each test construct at five different pH’s. Finally, the OD600 was measured every five minutes for 24 hours while the plate was incubated at 37°C, shook for 1 minute before every sample and between every sample in a spectrophotometer.
Results
Works. Refer to: http://2012.igem.org/Team:Stanford-Brown/HellCell/pH
Applications of BBa_K847080
User Reviews
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