Difference between revisions of "Part:BBa K847040:Experience"
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After transforming bacteria with our test plasmid, we grew approximately equal amounts of liquid culture of the negative control (see Test Plasmid) in LB+amp at 15°C, the lower limit of ''E. coli''’s survival range. OD measurements were taken at time points t = 0 days and t = 9 days. | After transforming bacteria with our test plasmid, we grew approximately equal amounts of liquid culture of the negative control (see Test Plasmid) in LB+amp at 15°C, the lower limit of ''E. coli''’s survival range. OD measurements were taken at time points t = 0 days and t = 9 days. | ||
Revision as of 00:48, 4 October 2012
Assay
After transforming bacteria with our test plasmid, we grew approximately equal amounts of liquid culture of the negative control (see Test Plasmid) in LB+amp at 15°C, the lower limit of E. coli’s survival range. OD measurements were taken at time points t = 0 days and t = 9 days.
Results
Works for desiccation resistance. Refer to: http://2012.igem.org/Team:Stanford-Brown/HellCell/Desiccation Further testing needed for cold resistance. Refer to: http://2012.igem.org/Team:Stanford-Brown/HellCell/Cold
Applications of BBa_K847040
Astrobiology revolves around three central questions: "Where do we come from?", "Where are we going?", and "Are we alone?" To approach the second question, the Hell Cell subgroup of the Stanford-Brown iGEM team developed BioBricks that allow a cell to survive harsh extraterrestrial conditions. Such a toolset could create a space-ready synthetic organism to perform useful functions off-world. This gene is one of the toolset, potentially conferring cold and desiccation resistance to otherwise intolerant bacteria.
User Reviews
UNIQ4249fdf00a655fa4-partinfo-00000000-QINU UNIQ4249fdf00a655fa4-partinfo-00000001-QINU