Difference between revisions of "Part:BBa K847040:Design"

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===References===
 
===References===
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Chattopadhyay, M. K. (2006). Mechanism of bacterial adaptation to low temperature. ''J. Biosci., 31'' (1), 157-165.
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Bonaterra, A., Camps, J., Montesinos, E. (2005). Osmotically induced trehalose and glycine betaine accumulation improves tolerance to desiccation, survival and efficacy of the postharvest biocontrol agent ''Pantoea agglomerans'' EPS125. ''FEMS Microbiol. Lett., 250'': 1-8.
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Cayley, S., Lewis, B. A., Record Jr., T. (1992). Origins of the osmoprotective properties of betaine and proline in ''Escherichia coli'' K-12. ''J. Bacteriol. 174''(5): 1586-1595.

Latest revision as of 00:43, 4 October 2012

''Escherichia coli'' choline dehydrogenase (betA)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 952
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 952
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 952
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 952
    Illegal NgoMIV site found at 28
    Illegal NgoMIV site found at 37
    Illegal AgeI site found at 930
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was isolated via colony PCR from Escherichia coli. It does not contain promoter, RBS, transcriptional terminator, etc. These elements must be connected to this part for proper functionality.

Source

Escherichia coli

References

Chattopadhyay, M. K. (2006). Mechanism of bacterial adaptation to low temperature. J. Biosci., 31 (1), 157-165.

Bonaterra, A., Camps, J., Montesinos, E. (2005). Osmotically induced trehalose and glycine betaine accumulation improves tolerance to desiccation, survival and efficacy of the postharvest biocontrol agent Pantoea agglomerans EPS125. FEMS Microbiol. Lett., 250: 1-8.

Cayley, S., Lewis, B. A., Record Jr., T. (1992). Origins of the osmoprotective properties of betaine and proline in Escherichia coli K-12. J. Bacteriol. 174(5): 1586-1595.