Difference between revisions of "Part:BBa K781000"

Revision as of 00:27, 4 October 2012

[R0010][B0034] - E. coli K12 FliC

This is the complete flagellin coding sequence with biobrick standard cut sites removed, under the promoter R0010 and RBS B0034. This part can be used to over express the flagellin monomer.

The main use of this part is for PCR overlap extension, which will make an insertion at the annotated site, given that the insert is in the appropriate format. The resulting chimera will express protein immediately after the PCR reaction is transformed.

This part has been previously characterized as BBa_K777109. The part that we have submitted has some slight differences in the silent mutations made to remove Biobrick cut sites. This part also includes the promoter R0010 and the RBS B0034.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1452
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 529
Illegal AgeI site found at 937
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K781000 short</partinfo>
-This is the complete flagellin coding sequence with biobrick standard cut sites removed, under the promoter R0010 and RBS B0034. This part can be used to over express the flagellin monomer. Or, this part can be used as a template for PCR overlap extension, which will make an insertion at the annotated site, given that the insert is in the appropriate format. The resulting chimera will express protein immediately after the PCR reaction is transformed.
+This is the complete flagellin coding sequence with biobrick standard cut sites removed, under the promoter R0010 and RBS B0034. This part can be used to over express the flagellin monomer.
+The main use of this part is for PCR overlap extension, which will make an insertion at the annotated site, given that the insert is in the appropriate format. The resulting chimera will express protein immediately after the PCR reaction is transformed.
+This part has been previously characterized as BBa_K777109. The part that we have submitted has some slight differences in the silent mutations made to remove Biobrick cut sites. This part also includes the promoter R0010 and the RBS B0034.
 <!-- Add more about the biology of this part here