Difference between revisions of "Part:BBa K773002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | We codon optimized the gene for E. coli and eliminated four restriction sites. | + | We codon optimized the gene for E. coli and eliminated four restriction sites. Our characterization assays of this part were inconclusive, but the sequencing data shows that this is proteorhodopsin. To view the characterization assay view [http://2012.igem.org/Team:Caltech/Parts/Characterization this page]. |
Line 12: | Line 12: | ||
===Source=== | ===Source=== | ||
− | This part was synthesized by Daisy Lin, Chenxi Qiu, Emzo de los Santos, Nate Glasser, | + | This part was synthesized by Daisy Lin, Chenxi Qiu, Emzo de los Santos, Nate Glasser, Edward Pursifull, and Katie Knister with [http://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#Gene_Assembly gene assembly] using the information from the paper [http://www.pnas.org/content/104/7/2408.abstract Light-powering Escherichia coli with proteorhodopsin]. |
===References=== | ===References=== |
Revision as of 00:21, 4 October 2012
Proteorhodopsin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 515
Illegal XhoI site found at 88 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 277
Illegal AgeI site found at 652 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We codon optimized the gene for E. coli and eliminated four restriction sites. Our characterization assays of this part were inconclusive, but the sequencing data shows that this is proteorhodopsin. To view the characterization assay view [http://2012.igem.org/Team:Caltech/Parts/Characterization this page].
Source
This part was synthesized by Daisy Lin, Chenxi Qiu, Emzo de los Santos, Nate Glasser, Edward Pursifull, and Katie Knister with [http://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#Gene_Assembly gene assembly] using the information from the paper [http://www.pnas.org/content/104/7/2408.abstract Light-powering Escherichia coli with proteorhodopsin].
References
[http://www.pnas.org/content/104/7/2408.abstract Light-powering Escherichia coli with proteorhodopsin]
[http://helixweb.nih.gov/dnaworks/ DNAWorks] (to obtain oligonucleotides to assemble the gene)