Difference between revisions of "Part:BBa K847000:Experience"
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| − | This experience page is provided so that any user may enter their experience using this part.<BR> | + | This experience page is provided so that any user may enter their experience using this part.<BR> |
| − | + | '''Assay''' | |
| + | Liquid cultures of NEB5α E. coli transformed with MntH, recA, sod Cu/Zn, Dps 1 (Sod Mn and Dps 2 constructs could not be completely assembled in time), and negative control were grown up over night at 37°C. The following day, the cells were washed and resuspended in 0.9% NaCl solution. Cell concentration was then adjusted to 10^7/mL in 5mL of a glass Petri dish. Each sample was then exposed to 1.2 J/(m^2*sec) of UV-C radiation from a UV lamp for a cumulative of 0 seconds, 2 seconds, 5 seconds, 10 seconds, 20 seconds, and 30 seconds. After each exposure, a dilution spot assay was conducted to determine the final number of surviving cells. | ||
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| + | '''Results''' | ||
| + | Further testing needed. Refer to http://2012.igem.org/Team:Stanford-Brown/HellCell/Radiation. | ||
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===Applications of BBa_K847000=== | ===Applications of BBa_K847000=== | ||
Revision as of 00:20, 4 October 2012
This experience page is provided so that any user may enter their experience using this part.
Assay
Liquid cultures of NEB5α E. coli transformed with MntH, recA, sod Cu/Zn, Dps 1 (Sod Mn and Dps 2 constructs could not be completely assembled in time), and negative control were grown up over night at 37°C. The following day, the cells were washed and resuspended in 0.9% NaCl solution. Cell concentration was then adjusted to 10^7/mL in 5mL of a glass Petri dish. Each sample was then exposed to 1.2 J/(m^2*sec) of UV-C radiation from a UV lamp for a cumulative of 0 seconds, 2 seconds, 5 seconds, 10 seconds, 20 seconds, and 30 seconds. After each exposure, a dilution spot assay was conducted to determine the final number of surviving cells.
Results
Further testing needed. Refer to http://2012.igem.org/Team:Stanford-Brown/HellCell/Radiation.
Applications of BBa_K847000
Astrobiology revolves around three central questions: "Where do we come from?", "Where are we going?", and "Are we alone?" To approach the second question, the Hell Cell subgroup of the Stanford-Brown iGEM team developed BioBricks that allow a cell to survive harsh extraterrestrial conditions. Such a toolset could create a space-ready synthetic organism to perform useful functions off-world. This gene is one of the toolset, potentially conferring radiation resistance to otherwise radiation-intolerant bacteria.
User Reviews
UNIQ419851e13eeb81e7-partinfo-00000000-QINU UNIQ419851e13eeb81e7-partinfo-00000001-QINU