Difference between revisions of "Part:BBa K892009"

Revision as of 23:28, 3 October 2012

fucO

The gene fucO, which codes for glycolaldehyde reductase, is one of the genes required for E. coli to utilize ethylene glycol as a food source. The coding sequence is put behind a strong biofab promoter and RBS. This part should be used with aldA for the desired effect.

Usage and Biology

This part was characterized by [http://2012.igem.org/Team:Washington Washington 2012] using the turbidostat. MG1655 cells were cotransformed with fucO and aldA, the assay is described on the [http://2012.igem.org/Team:Washington/Protocols/EG_Assay wiki] and some of the data is shown below.

M9 30 mM ethylene glycol media injected into the culture vessel every minute to maintain the arbitrary optical density of 0.3.

Optical density measurements of E. coli transformed with fucO pGA3K3 + aldA pGA1C3 within the turbidostat growing on M9 30 mM ethylene glycol media.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 251
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 251
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 251
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 251
Illegal AgeI site found at 967
Illegal AgeI site found at 1168
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 [[Image:Washington_Turbidostat_OD.png|border|450px|right|thumb|Optical density measurements of <i>E. coli</i> transformed with fucO pGA3K3 + aldA pGA1C3 within the turbidostat growing on M9 30 mM ethylene glycol media.]]
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 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K892009 SequenceAndFeatures</partinfo>