Difference between revisions of "Part:BBa K549019:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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<partinfo>BBa_J70044 AddReview number</partinfo>
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<I>2012 Utah_State iGEM Team</I>
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The 2012 Utah_State iGEM Team has improved upon this part with [https://parts.igem.org/wiki/index.php/Part:BBa_K844000 BBa_K844000].
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Translational analysis of BBa_J70044 shows the amino acid sequence that it codes for is:
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I R G R F Stop Met A G H H H H H H H H H H T G Stop
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This sequence contains an upstream stop codon which if included as part of a fusion protein would prevent the 10x-His tag from even being incorporated into the protein; this part also includes many additional amino acid codons not necessary to His-tag function.
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BBa_K844000 improves on this part by removing the upstream stop codon, removing the additional amino acid codons residues upstream and downstream of the His Tag, and adding an additional stop codon downstream to ensure protein termination. BBa_K844000 is also readily compatible with the [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] cloning system.
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Latest revision as of 22:54, 3 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K549019

User Reviews

UNIQ2b85fa3190119f31-partinfo-00000000-QINU

No review score entered. 2012 Utah_State iGEM Team

The 2012 Utah_State iGEM Team has improved upon this part with BBa_K844000.

Translational analysis of BBa_J70044 shows the amino acid sequence that it codes for is:

I R G R F Stop Met A G H H H H H H H H H H T G Stop

This sequence contains an upstream stop codon which if included as part of a fusion protein would prevent the 10x-His tag from even being incorporated into the protein; this part also includes many additional amino acid codons not necessary to His-tag function.

BBa_K844000 improves on this part by removing the upstream stop codon, removing the additional amino acid codons residues upstream and downstream of the His Tag, and adding an additional stop codon downstream to ensure protein termination. BBa_K844000 is also readily compatible with the Assembly Standard #23 (Silver Fusion) cloning system.

UNIQ2b85fa3190119f31-partinfo-00000002-QINU