Difference between revisions of "Part:BBa K112703:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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| + | {|width='80%' style='border:1px solid gray' | ||
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| + | <partinfo>BBa_J70044 AddReview number</partinfo> | ||
| + | <I>2012 Utah_State iGEM Team</I> | ||
| + | |width='60%' valign='top'| | ||
| + | The 2012 Utah_State iGEM Team has improved upon this part with [https://parts.igem.org/wiki/index.php/Part:BBa_K844000 BBa_K844000]. BBa_K844000 improves on this part by removing the upstream Met (ATG) codon, extending the His repeat region to 10x (which improves binding affinity and purity of extracted protein product), and adding the double stop (TAATAA) downstream of the His tag to ensure protein termination, without the need for an additional cloning step for the stop codon. BBa_K844000 is also readily compatible with the [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] | ||
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Latest revision as of 22:49, 3 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K112703
User Reviews
UNIQf790ca189f3b6044-partinfo-00000000-QINU
|
No review score entered. 2012 Utah_State iGEM Team |
The 2012 Utah_State iGEM Team has improved upon this part with BBa_K844000. BBa_K844000 improves on this part by removing the upstream Met (ATG) codon, extending the His repeat region to 10x (which improves binding affinity and purity of extracted protein product), and adding the double stop (TAATAA) downstream of the His tag to ensure protein termination, without the need for an additional cloning step for the stop codon. BBa_K844000 is also readily compatible with the Assembly Standard #23 (Silver Fusion) |
UNIQf790ca189f3b6044-partinfo-00000002-QINU